| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.054638 |
| Chromosome: | chromosome 16 |
| Location: | 6767186 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g673729 | CPN11 | Chaperonin 11, chloroplastic; (1 of 1) PTHR10772:SF13 - CHLOROPLAST CHAPERONIN 10 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGTCGTGCCCCAAACGCGCACTCCAGCT |
| Internal bar code: | CGGCAATGTCTGAGTCTGACAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 439 |
| LEAP-Seq percent confirming: | 99.6822 |
| LEAP-Seq n confirming: | 941 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTCATTTCGAAGAATGGCG |
| Suggested primer 2: | CCAAGACTGCGCTGTTGTAA |