| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.054647 |
| Chromosome: | chromosome 5 |
| Location: | 1975926 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g241640 | (1 of 1) IPR000253//IPR000859//IPR014710 - Forkhead-associated (FHA) domain // CUB domain // RmlC-like jelly roll fold | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTAGAAAGCAGCTGGGGCCCGATGTACT |
| Internal bar code: | GATGGACGGGGCTGGGCAACAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 687 |
| LEAP-Seq percent confirming: | 92.8312 |
| LEAP-Seq n confirming: | 9453 |
| LEAP-Seq n nonconfirming: | 730 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCACCAGACCAGACGAGT |
| Suggested primer 2: | ACCCTGTGTGATCCTCTTCG |