| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.054686 |
| Chromosome: | chromosome 3 |
| Location: | 9148709 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g212081 | (1 of 3) IPR013328 - 6-phosphogluconate dehydrogenase, domain 2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCACAGCGACAGGGACCGCCTTCACGCT |
| Internal bar code: | TGGAAGTCCCGACGTTGTTCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1183 |
| LEAP-Seq percent confirming: | 99.6485 |
| LEAP-Seq n confirming: | 3969 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGATGAGCTTCAGGCAACAC |
| Suggested primer 2: | TACCATCTGCCCAACTCTCC |