| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.054743 |
| Chromosome: | chromosome 5 |
| Location: | 1387587 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g244300 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGGGACGAATGTGTCCAGCTGATCAATG |
| Internal bar code: | GCGCACCCTGGCCCGGGTGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 893 |
| LEAP-Seq percent confirming: | 98.686 |
| LEAP-Seq n confirming: | 27338 |
| LEAP-Seq n nonconfirming: | 364 |
| LEAP-Seq n unique pos: | 143 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCATGTATGTACGCTGGC |
| Suggested primer 2: | GCGAAGGAGAAAGAGAGGGT |