| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.054748 |
| Chromosome: | chromosome 6 |
| Location: | 3197413 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g275950 | FAP395 | Flagellar Associated Protein 395 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTACATACGTGATTGTACAACGAACCCCAC |
| Internal bar code: | TGCGCAGCCCCCAACGAGGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 265 |
| LEAP-Seq percent confirming: | 90.625 |
| LEAP-Seq n confirming: | 58 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTAACTGAACCGAACCCA |
| Suggested primer 2: | GGCTAGTGTCTACCCCCACA |