Insertion junction: LMJ.RY0402.054751_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.054751
Chromosome:chromosome 17
Location:2695169
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre17.g717900 PHC1 (1 of 24) IPR003882//IPR024616 - Pistil-specific extensin-like protein // Pherophorin; Pherophorin-chlamydomonas homolog 1 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCAAGACCGACATGCTGCTCAAGGCCGAGT
Internal bar code:GTTCCGATTGTACAGTGTTCAT

Confirmation - LEAP-Seq

LEAP-Seq distance:4
LEAP-Seq percent confirming:19.0476
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:17
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GAGGCCTGCTTATGTTGCTC
Suggested primer 2:TTTGACAGCCAGAACGACTG