| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.054759 |
| Chromosome: | chromosome 3 |
| Location: | 3006211 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g163850 | (1 of 1) K10638 - E3 ubiquitin-protein ligase UHRF1 [EC:6.3.2.19] (UHRF1, NP95) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCGGCACTGCATGTGGGGGGTGCAGGTG |
| Internal bar code: | TAAGAGTCGATGGCACTCTTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 220 |
| LEAP-Seq percent confirming: | 99.8277 |
| LEAP-Seq n confirming: | 1738 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAAGGTGATACGGCGAGGTC |
| Suggested primer 2: | CCGGACAACACGTGATACAG |