Insertion junction: LMJ.RY0402.054763_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.054763
Chromosome:chromosome 17
Location:2238925
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g713600 SUB13 (1 of 14) 3.4.21.62 - Subtilisin; Subtilisin-like protease 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGCAAGCTCAGCCTGTACGGCTGCACCCA
Internal bar code:TGGGGCTCGAATGCGGGCCCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:914
LEAP-Seq percent confirming:98.1452
LEAP-Seq n confirming:3122
LEAP-Seq n nonconfirming:59
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:GAATTGTTTTCGCCTTTCCA
Suggested primer 2:AGCGTTTGCACCTCTTCCTA