| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.054766 |
| Chromosome: | chromosome 2 |
| Location: | 1563538 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g084800 | SPO11A | (1 of 1) K10878 - meiotic recombination protein SPO11 (SPO11); meoisis-related DNA topoisomerase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTACGAACATGTGCACACAGAGACACATTC |
| Internal bar code: | ATAACGCGCGCAAACCCGCCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 738 |
| LEAP-Seq percent confirming: | 13.3014 |
| LEAP-Seq n confirming: | 139 |
| LEAP-Seq n nonconfirming: | 906 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATTTATAACCCCCGCCTCC |
| Suggested primer 2: | CTGAGCTGTTTGGAAGGAGG |