Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.054767 |
Chromosome: | chromosome_6 |
Location: | 7002462 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre06.g296700 | HYDG1,HYDG | Hydrogenase assembly factor/biotin synthase | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GACTGCCCAACCTGTGCAGTAACGTATGTA |
Internal bar code: | TCGCACAAGCCGACATGCAGTA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 883 |
LEAP-Seq percent confirming: | 98.558 |
LEAP-Seq n confirming: | 6083 |
LEAP-Seq n nonconfirming: | 89 |
LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TCCAACAAGATTACCCTGGC |
Suggested primer 2: | ACTAGCCAACCCCGAAGTTT |