| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.054768 |
| Chromosome: | chromosome 10 |
| Location: | 4588826 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g452600 | (1 of 1) PTHR33911:SF1 - RRNA-PROCESSING PROTEIN EFG1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCTGGTCCTCAGGGGCACCCCTGCTGGTC |
| Internal bar code: | GCAGTGCACACGTCGTGCCGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 275 |
| LEAP-Seq percent confirming: | 99.6989 |
| LEAP-Seq n confirming: | 1987 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCATGCACTTCCTAGATGC |
| Suggested primer 2: | GAGGAGGACGATACGGATGA |