| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.054769 |
| Chromosome: | chromosome 16 |
| Location: | 6607550 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g672100 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGGGCTGCAGCGGCCGGCGCGTGCGCCTC |
| Internal bar code: | GTGACTGCGTGGCTGCCCGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 417 |
| LEAP-Seq percent confirming: | 39.3384 |
| LEAP-Seq n confirming: | 4911 |
| LEAP-Seq n nonconfirming: | 7573 |
| LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCCAGTCCCAAACTCAACC |
| Suggested primer 2: | GACCTGGAAATGAAACGGAA |