Insertion junction: LMJ.RY0402.054770_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.054770
Chromosome:chromosome 9
Location:1668556
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g397050 (1 of 28) PTHR31600:SF2 - TINY MACROCYSTS PROTEIN B-RELATED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCAGCAGTAAAGTACAAGAAACACGACCTA
Internal bar code:TCTGATGATGGAACGGCGTCAG

Confirmation - LEAP-Seq

LEAP-Seq distance:857
LEAP-Seq percent confirming:99.0606
LEAP-Seq n confirming:2109
LEAP-Seq n nonconfirming:20
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:ATCAGGTTGTGTGCGTGTGT
Suggested primer 2:CGAATGTTGAGTGGTTGGTG