Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.054793 |
Chromosome: | chromosome 14 |
Location: | 3709105 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre14.g631950 | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCACATGTAGAGTACCCTTATTACCTGGC |
Internal bar code: | ATGGGTGCTGTCCTCACGAGAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 902 |
LEAP-Seq percent confirming: | 99.0862 |
LEAP-Seq n confirming: | 3470 |
LEAP-Seq n nonconfirming: | 32 |
LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTGTGTCCATCCGTGTCTTG |
Suggested primer 2: | CCTGCTTCTGACGAGTAGGG |