| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.054799 |
| Chromosome: | chromosome_7 |
| Location: | 5176550 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre07.g348400 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AGGAGGAGGCGTCGAGGCAGGGCACCAGTA |
| Internal bar code: | CCAGACGCCCTTCCATGGCTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 340 |
| LEAP-Seq percent confirming: | 70.2055 |
| LEAP-Seq n confirming: | 205 |
| LEAP-Seq n nonconfirming: | 87 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACGGCAGTGAAGAGAGGC |
| Suggested primer 2: | AGAGGCATGACGAGTGTGTG |