Insertion junction: LMJ.RY0402.054874_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.054874
Chromosome:chromosome 10
Location:795353
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre10.g423200 (1 of 66) 2.7.12.1 - Dual-specificity kinase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACCACCCCTCTGCCTCATAGGTCAAAGCG
Internal bar code:CGTCGGCTGCGAATAATGGATC

Confirmation - LEAP-Seq

LEAP-Seq distance:940
LEAP-Seq percent confirming:99.845
LEAP-Seq n confirming:1288
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GACGTTGAGTTGTCTTGGCA
Suggested primer 2:CAAGGACTTGCCCAAATTGT