| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.054893 |
| Chromosome: | chromosome 17 |
| Location: | 426183 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g698903 | (1 of 1) IPR000014//IPR002395 - PAS domain // HMW kininogen | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACCACCGCCACTGCTGCCATCACCCGTGC |
| Internal bar code: | AACGAATACTATAGAGCCCTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1294 |
| LEAP-Seq percent confirming: | 99.6077 |
| LEAP-Seq n confirming: | 3301 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTACGTTCAGGAGCAAAGCC |
| Suggested primer 2: | AAGTCGGGATGCAAACATTC |