| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.054898 |
| Chromosome: | chromosome 16 |
| Location: | 6286757 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g674650 | (1 of 1) 3.5.1.59 - N-carbamoylsarcosine amidase / N-carbamoylsarcosine amidohydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACACAGGGAGTGCGCACTGCTGTCCGCAA |
| Internal bar code: | CGGGGATGGGCAAACAATTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 940 |
| LEAP-Seq percent confirming: | 99.5095 |
| LEAP-Seq n confirming: | 1826 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCCCTGGTGTTCAGATGAG |
| Suggested primer 2: | CCAGAGGCTGCTACTGTTCC |