| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.054898 |
| Chromosome: | chromosome 16 |
| Location: | 6286759 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g674650 | (1 of 1) 3.5.1.59 - N-carbamoylsarcosine amidase / N-carbamoylsarcosine amidohydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGTGGCAAGGCATGTGCCCCTGCCCTCC |
| Internal bar code: | CGAACCGTACCCGGCAAGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 939 |
| LEAP-Seq percent confirming: | 99.8911 |
| LEAP-Seq n confirming: | 917 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCCCTGGTGTTCAGATGAG |
| Suggested primer 2: | CCAGAGGCTGCTACTGTTCC |