| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.054902 |
| Chromosome: | chromosome_9 |
| Location: | 6237693 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g406300 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTTCCAGGGGGTTCTACTCCCTACGGACGA |
| Internal bar code: | GGACTAAGATTGACGGTGGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 193 |
| LEAP-Seq percent confirming: | 90.1861 |
| LEAP-Seq n confirming: | 533 |
| LEAP-Seq n nonconfirming: | 58 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGTAGTCGTACGCGGTG |
| Suggested primer 2: | CTGCGAAGTCTTTAAACCGC |