| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.054909 |
| Chromosome: | chromosome 13 |
| Location: | 3161853 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g585250 | (1 of 1) PF08005 - PHR domain (PHR) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCAGGCTCGCTCAACTTTCAGACGCTTAT |
| Internal bar code: | CTGCCAGGGGAATAACAGGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 480 |
| LEAP-Seq percent confirming: | 97.3983 |
| LEAP-Seq n confirming: | 7899 |
| LEAP-Seq n nonconfirming: | 211 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTCACAAGCAGCTGTCAAA |
| Suggested primer 2: | ACACACCGCCTGTAGTACCC |