| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.054909 |
| Chromosome: | chromosome 13 |
| Location: | 3161853 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g585250 | (1 of 1) PF08005 - PHR domain (PHR) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGAAACGCCCCAAAGCGGCATCCATCTTC |
| Internal bar code: | ACCTCTGATCCTCATATATCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1076 |
| LEAP-Seq percent confirming: | 99.4385 |
| LEAP-Seq n confirming: | 5490 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTCACAAGCAGCTGTCAAA |
| Suggested primer 2: | ACACACCGCCTGTAGTACCC |