| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.054913 |
| Chromosome: | chromosome 4 |
| Location: | 1974843 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g218526 | (1 of 88) IPR011333 - POZ domain | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCCACAACACCGCGGGAAGCACTGCGGA |
| Internal bar code: | GCCATCGAGGTCCCGTTCGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 641 |
| LEAP-Seq percent confirming: | 99.0776 |
| LEAP-Seq n confirming: | 5156 |
| LEAP-Seq n nonconfirming: | 48 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAATGTTGTGTGCGTGATG |
| Suggested primer 2: | CATTAACGCCCTTCTTTGGA |