Insertion junction: LMJ.RY0402.054913_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.054913
Chromosome:chromosome 4
Location:1974854
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre04.g218526 (1 of 88) IPR011333 - POZ domain 5'UTR_intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGAACGGGGGGGGGGGGGGGCGCCTCACT
Internal bar code:TGAGTAAACGGCAAGTGCGGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:935
LEAP-Seq percent confirming:99.6709
LEAP-Seq n confirming:2423
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:TGAATGTTGTGTGCGTGATG
Suggested primer 2:CATTAACGCCCTTCTTTGGA