Insertion junction: LMJ.RY0402.054916_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.054916
Chromosome:chromosome 7
Location:2959051
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g332901 (1 of 80) IPR003882 - Pistil-specific extensin-like protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGCTGACGTCCGGCAGTGTGCACAGCCGG
Internal bar code:CCCATCAACTCCGAGAGCGAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:832
LEAP-Seq percent confirming:95.4357
LEAP-Seq n confirming:690
LEAP-Seq n nonconfirming:33
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:CCATAACCTTCAATCCCCCT
Suggested primer 2:CAGTGGAGCCCCTACTAACG