| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.054934 |
| Chromosome: | chromosome 4 |
| Location: | 2165352 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g219750 | HEL22 | (1 of 1) PTHR13710//PTHR13710:SF94 - DNA HELICASE RECQ FAMILY MEMBER // ATP-DEPENDENT DNA HELICASE RECQ FAMILY PROTEIN; DEAD/DEAH box helicase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGGACGGTCAGGGTCTCTGGGCCGCCCGG |
| Internal bar code: | TGCGATCTTCACTTTATTAATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 272 |
| LEAP-Seq percent confirming: | 99.4809 |
| LEAP-Seq n confirming: | 1533 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCGTCTTCAGTGCATCCTG |
| Suggested primer 2: | TTGTGCATGTGGAGGAATGT |