| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.054959 |
| Chromosome: | chromosome 12 |
| Location: | 1159455 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g490050 | TPT18,GMT1,TPT19 | (1 of 3) K15356 - GDP-mannose transporter (VRG4, GONST1); Putative GDP-mannose transporter | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCCGTGTGCTTGCGTCCCACAGCTTCAG |
| Internal bar code: | CTCGAGTTTGCCTATTGTACCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 314 |
| LEAP-Seq percent confirming: | 99.7677 |
| LEAP-Seq n confirming: | 1718 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATGCTGGCATCTAACTGA |
| Suggested primer 2: | GGCGAATAACGGCTTAAACA |