| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.054960 |
| Chromosome: | chromosome 9 |
| Location: | 7301725 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g412803 | CGL88 | predicted protein; (1 of 1) PTHR10383//PTHR10383:SF24 - SERINE INCORPORATOR // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGGCCCGATGGCCCCGGCGGGTCCGGCA |
| Internal bar code: | GGGTGTAACAAGAAACAAGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 71 |
| LEAP-Seq percent confirming: | 94.0295 |
| LEAP-Seq n confirming: | 7780 |
| LEAP-Seq n nonconfirming: | 494 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGACTCTTGGGTCCCACTC |
| Suggested primer 2: | GGGATTGAGTGGTTGCTTGT |