| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.054991 |
| Chromosome: | chromosome 1 |
| Location: | 5342260 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g037500 | (1 of 11) IPR000626//IPR029071 - Ubiquitin domain // Ubiquitin-related domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCAGCAACTGCGACGACGGTCCGATGCA |
| Internal bar code: | GCTGCCATGCACAGATTAAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1004 |
| LEAP-Seq percent confirming: | 99.4243 |
| LEAP-Seq n confirming: | 2763 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCGTGGCCTTTTCAGAGTT |
| Suggested primer 2: | TGTGAGACATACGGTGCCAT |