| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.055002 |
| Chromosome: | chromosome 3 |
| Location: | 5577884 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g186650 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTAGGCGTTGGAAGATTGCTAGGCCGTGA |
| Internal bar code: | GGTCTTACTAGGGGTAAAGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1202 |
| LEAP-Seq percent confirming: | 99.707 |
| LEAP-Seq n confirming: | 22458 |
| LEAP-Seq n nonconfirming: | 66 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAGCTGCTTTGTGGCAAAT |
| Suggested primer 2: | GACTCCTCTATGCGTGCCTC |