Insertion junction: LMJ.RY0402.055026_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.055026
Chromosome:chromosome 16
Location:5950022
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g676900 UAA5 UDP-galactose transporter; (1 of 2) K15276 - solute carrier family 35 (adenosine 3'-phospho 5'-phosphosulfate transporter), member B2 (SLC35B2, PAPST1) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGTCGAAACACTGTCCGAGATACGGTGGT
Internal bar code:CGGTCTTCAACCGGGCCATCTA

Confirmation - LEAP-Seq

LEAP-Seq distance:837
LEAP-Seq percent confirming:97.3888
LEAP-Seq n confirming:2014
LEAP-Seq n nonconfirming:54
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:ATGTTAGCAGCCATAACCCG
Suggested primer 2:ACACGCACACACACACACAC