| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.055027 |
| Chromosome: | chromosome 8 |
| Location: | 1169380 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g362950 | (1 of 3) IPR002202 - Hydroxymethylglutaryl-CoA reductase, class I/II | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTTTGAAAGGAACTCTTGCCTGTGTGGCC |
| Internal bar code: | TCTGGAGCGGATAGAAGCTTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 309 |
| LEAP-Seq percent confirming: | 99.6845 |
| LEAP-Seq n confirming: | 2528 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTACACACCCTGCTATGC |
| Suggested primer 2: | ACCGGCACATCCAGTAACTC |