Insertion junction: LMJ.RY0402.055030_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.055030
Chromosome:chromosome 3
Location:4297969
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g174672 (1 of 1) 3.1.1.93 - Mycophenolic acid acyl-glucuronide esterase / Mycophenolic acid acyl-glucuronide deglucuronidase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGTGAAGTAGGCGGGGCCCACCCAGTCAC
Internal bar code:GTCTCGTCGCCGGTTAGTAGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:471
LEAP-Seq percent confirming:99.7596
LEAP-Seq n confirming:830
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CACACTTTCACTCACCCCCT
Suggested primer 2:CACCTAAACCTTCCGTGCAT