| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.055053 |
| Chromosome: | chromosome 17 |
| Location: | 1080555 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g703850 | DC1,ODA3,ODA-DC1,DCC1 | Outer Arm Dynein Docking Complex 1 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGAGGCAGTGCCGAGGCGGTAGTGGGTAC |
| Internal bar code: | AACGGTTTGTAACATGCAAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 342 |
| LEAP-Seq percent confirming: | 99.6268 |
| LEAP-Seq n confirming: | 6941 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGCTCCTCGTCTATTTGC |
| Suggested primer 2: | ACCTTATCGGTGGACACTGC |