| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.055057 |
| Chromosome: | chromosome 17 |
| Location: | 3177099 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g721850 | SSA16 | cilia-sensing, structure and/or assembly; (1 of 1) K08292 - elongation factor 2 kinase (EEF2K) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTTTCGCCCGAGATCGTGACAGTTAAGTA |
| Internal bar code: | AGTTATGGTACAAAGTTTCATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 971 |
| LEAP-Seq percent confirming: | 98.4711 |
| LEAP-Seq n confirming: | 5990 |
| LEAP-Seq n nonconfirming: | 93 |
| LEAP-Seq n unique pos: | 47 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTATGCGACCTTCCCTGT |
| Suggested primer 2: | AAAACGACCTGTATGCCTGG |