| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.055094 |
| Chromosome: | chromosome 5 |
| Location: | 867204 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g247650 | (1 of 32) IPR000536 - Nuclear hormone receptor, ligand-binding domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGTCGCAGCAGCCCCACCACTTTCAGCA |
| Internal bar code: | TAGGAAGGCAAGAGGCAGCCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 236 |
| LEAP-Seq percent confirming: | 61.1888 |
| LEAP-Seq n confirming: | 2100 |
| LEAP-Seq n nonconfirming: | 1332 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTGTGCATGTGTGTACCG |
| Suggested primer 2: | AGCCATGTACCGTTACGGAG |