| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.055109 |
| Chromosome: | chromosome 11 |
| Location: | 602217 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467611 | (1 of 10) PF01062 - Bestrophin, RFP-TM, chloride channel (Bestrophin) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAACGCCATCACCACCTGATGGGGAGCGAG |
| Internal bar code: | GGTGGCGTGTGCGAGCTAATAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 711 |
| LEAP-Seq percent confirming: | 66.3784 |
| LEAP-Seq n confirming: | 5986 |
| LEAP-Seq n nonconfirming: | 3032 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGTGTGTAGGGAGGGACA |
| Suggested primer 2: | GTGTATGGGTTTGGGTGTCC |