| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.055141 |
| Chromosome: | chromosome 10 |
| Location: | 4560141 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g452400 | CSF1 | Chlamydomonas specific family F protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTCGGACACTCGCGGTTGTGTCACGTCCG |
| Internal bar code: | GGCGCCGTCGCGGTATACTCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 768 |
| LEAP-Seq percent confirming: | 98.4289 |
| LEAP-Seq n confirming: | 3383 |
| LEAP-Seq n nonconfirming: | 54 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGAGCTTTAGGTGCTTTTG |
| Suggested primer 2: | TCTACGTCGCAGGACATCTG |