Insertion junction: LMJ.RY0402.055149_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.055149
Chromosome:chromosome 2
Location:1699685
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g086000 ARS8 Arylsulfatase; (1 of 1) IPR002591//IPR003882//IPR017850 - Type I phosphodiesterase/nucleotide pyrophosphatase/phosphate transferase // Pistil-specific extensin-like protein // Alkaline-phosphatase-like, core domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGGTATAGCAAGAGGGGCATGGCGTGCTG
Internal bar code:ACCAAGGAACTGAATGTCTCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:769
LEAP-Seq percent confirming:99.5423
LEAP-Seq n confirming:2827
LEAP-Seq n nonconfirming:13
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:TTGACCTCCCACATCATTCA
Suggested primer 2:CAATCAGAAACGTTCGAGCA