Insertion junction: LMJ.RY0402.055149_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.055149
Chromosome:chromosome 2
Location:1699689
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g086000 ARS8 Arylsulfatase; (1 of 1) IPR002591//IPR003882//IPR017850 - Type I phosphodiesterase/nucleotide pyrophosphatase/phosphate transferase // Pistil-specific extensin-like protein // Alkaline-phosphatase-like, core domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCTTGCTACCGCGCCTGTCTCCTTTCCCA
Internal bar code:GATACAAAGGCCACGGATCTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1024
LEAP-Seq percent confirming:99.5106
LEAP-Seq n confirming:3253
LEAP-Seq n nonconfirming:16
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:TTGACCTCCCACATCATTCA
Suggested primer 2:CAATCAGAAACGTTCGAGCA