| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.055178 |
| Chromosome: | chromosome 7 |
| Location: | 3976143 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g339700 | SUF3,SUFC1,SUFC | Iron-sulfur cluster assembly protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGAATCCACCCACGCCGCTCAGCCACTCGT |
| Internal bar code: | CGTCTGGCCAGGCACAGAGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1021 |
| LEAP-Seq percent confirming: | 99.5344 |
| LEAP-Seq n confirming: | 6413 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAAGGTGTGCTGTGAAGAA |
| Suggested primer 2: | GTGTCCCCTACACTCCTCCA |