| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.055183 |
| Chromosome: | chromosome 1 |
| Location: | 2649075 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g015500 | CGL91 | (1 of 18) PTHR10774 - EXTENDED SYNAPTOTAGMIN-RELATED; Conserved in the Green Lineage | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTCTCCTCCCAGACCGGGTTCTTGCCGC |
| Internal bar code: | CTTTTTGAGGATGGTGTTTCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 887 |
| LEAP-Seq percent confirming: | 99.684 |
| LEAP-Seq n confirming: | 631 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGAGCGCTTTTGGTGACCT |
| Suggested primer 2: | GCAAGACTCTTCCATCTGCC |