Insertion junction: LMJ.RY0402.055204_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.055204
Chromosome:chromosome 16
Location:6290092
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g674600 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTGCCGCCGTCGCCGAGCGCGGTGGCGCC
Internal bar code:CATATATGAGCTTCCACGTGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:0.0
LEAP-Seq n confirming:0
LEAP-Seq n nonconfirming:23
LEAP-Seq n unique pos:0

Suggested primers for confirmation by PCR

Suggested primer 1:CCCTCTGAATGGAACTGGAA
Suggested primer 2:CTCTCATCCCTCTGTCTCCG