Insertion junction: LMJ.RY0402.055221_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.055221
Chromosome:chromosome 10
Location:4555016
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre10.g452350 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGCCATACATGAGCGCCCAAGTCCAAGAG
Internal bar code:TTAAATTTTTGCCAGAGGTTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:1142
LEAP-Seq percent confirming:99.5803
LEAP-Seq n confirming:14946
LEAP-Seq n nonconfirming:63
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:CACCCGCTTGTCCACTTAAT
Suggested primer 2:CTGACATCGCAAGATGCTGT