| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.055237 |
| Chromosome: | chromosome 4 |
| Location: | 104017 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g216650 | (1 of 1) PF01947 - Protein of unknown function (DUF98) (DUF98) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCCTGCCCTTCACCCAGGGTTGCCTAGT |
| Internal bar code: | AAATTAAGTAGGTTATAACGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 469 |
| LEAP-Seq percent confirming: | 95.6628 |
| LEAP-Seq n confirming: | 3507 |
| LEAP-Seq n nonconfirming: | 159 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTTGCGATACGGTACAGGC |
| Suggested primer 2: | TTTGGTTTAGTTTGGGCTGG |