| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.055238 |
| Chromosome: | chromosome 9 |
| Location: | 2742880 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g389400 | (1 of 1) K07007 - uncharacterized protein (K07007) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCTAGCCGCGCCCTGCTCTCGACACATT |
| Internal bar code: | GTGAAGGCCGGTCCGCGAGACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 712 |
| LEAP-Seq percent confirming: | 99.263 |
| LEAP-Seq n confirming: | 3502 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAAGGTCACACCGTATGT |
| Suggested primer 2: | TACCAACTCACCAAGCCTCC |