Insertion junction: LMJ.RY0402.055262_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.055262
Chromosome:chromosome 6
Location:8581309
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g308700 (1 of 1) PTHR31595:SF1 - LONG-CHAIN-ALCOHOL O-FATTY-ACYLTRANSFERASE 1-RELATED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCGATAGGATGTTCTGGTGGCATGGTACA
Internal bar code:CGTACCTGCGCCAGCTCTGGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:692
LEAP-Seq percent confirming:98.3264
LEAP-Seq n confirming:1880
LEAP-Seq n nonconfirming:32
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:CCTCTAGGCTCTGTGTTGCC
Suggested primer 2:TCGACACAGAGCTGATGACC