| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.055296 |
| Chromosome: | chromosome 1 |
| Location: | 827919 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g004200 | (1 of 44) IPR000048 - IQ motif, EF-hand binding site | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTGAGTACCGTACGGTATCCCGTGCCCGA |
| Internal bar code: | AAGCCGTCTCTTTCCCTGATAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 474 |
| LEAP-Seq percent confirming: | 95.6971 |
| LEAP-Seq n confirming: | 1112 |
| LEAP-Seq n nonconfirming: | 50 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCACACACTCCACACCCTA |
| Suggested primer 2: | ACCGATTTATGCGACACCAT |