| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.055300 |
| Chromosome: | chromosome 9 |
| Location: | 5784752 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g402034 | UBA4 | (1 of 1) 2.7.7.80//2.8.1.11 - Molybdopterin-synthase adenylyltransferase // Molybdopterin-synthase sulfurtransferase / Molybdopterin synthase sulfurylase; URM1 E1 activating enzyme | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCGGTCGTCACTGCCGCGCCTTGCAACGC |
| Internal bar code: | CGGAGTTAGAAGGCGAGTCCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 132 |
| LEAP-Seq percent confirming: | 99.7817 |
| LEAP-Seq n confirming: | 1828 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCGATCAACTACATGCCA |
| Suggested primer 2: | ACACACCTGTATGGTGGGGT |