Insertion junction: LMJ.RY0402.055303_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.055303
Chromosome:chromosome 9
Location:4517221
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g395917 (1 of 1) 2.3.1.32 - Lysine N-acetyltransferase / Lysine N(6)-acetyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCTCTGGAAGCCTGTTTCCAACGCCCAAC
Internal bar code:ACGTAACATAATATGATGACAG

Confirmation - LEAP-Seq

LEAP-Seq distance:557
LEAP-Seq percent confirming:99.3691
LEAP-Seq n confirming:1575
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:TTCAAGCAGCGTGTCAAATC
Suggested primer 2:CACGCACAATACTGAATGGG